Basic Medicine, Molecular and Cellular Biology, Genomics, Genetics and Epigenetics

Medical Biochemistry

To Understand the Principal Mechanisms that Underlie the Key Biological Processes of Differentiation, Cell Homeostasis, and Cellular Defense

Medical Sciences Course

  • Master / Doctoral Degree

Faculty

YAMAMOTO, MasayukiYAMAMOTO, Masayuki
YAMAMOTO, Masayuki

Professor, M.D. Ph.D.

*Concurrent Position

Research Theme

  • The mechanisms of cellular defense against oxidative stress that occurs via the Keap1-Nrf2 system
  • GATA1 and GATA2 regulation of erythroid and megakaryocytic cell differentiation
  • The transcriptional regulation of the erythropoietin gene
Research Keywords:

transcription factor, environmental response, hematopoietic differentiation, Keap1-Nrf2, GATA

Technical Keywords:

knockout mouse, transgenic mouse, cell culture, flow cytometry

Laboratory Introduction

The Human Genome Project recently revealed that our species has approximately thirty thousand genes. The individual identity of each cell is carefully scripted based a set of transcriptionally activated genes that occurs during differentiation. We regard this transcriptional gene regulation as one of the fundamental tenets accounting for the diversity and the mystery surrounding our own existence. Our personal goal as scientists is to decipher the principal mechanisms that underlie the key biological processes of differentiation, cell homeostasis, and cellular defense by elucidating the actions of these transcriptional gene pathways and networks. With each new discovery, we work towards increasing our understanding of human disease processes. The specific focus of our research is to illuminate mechanisms revolving around:
1) The mechanisms of cellular defense against oxidative stress that occurs via the Keap1-Nrf2 system
2) GATA1 and GATA2 regulation of erythroid and megakaryocytic cell differentiation
3) The transcriptional regulation of the erythropoietin gene

Figure 1. Molecular mechanism of Keap1/Nrf2 system

Figure 1. Molecular mechanism of Keap1/Nrf2 system

Figure 2. The function of GATA factors

Figure 2. The function of GATA factors

Recent Publications

  • Yamazaki H, et al. A remote GATA2 hematopoietic enhancer drives leukemogenesis in inv(3)(q21;q26) by activating EVI1 expression. Cancer Cell 25, 415-427 , 2014
  • Suzuki N, et al. Erythropoietin production in neuroepithelial and euralcrest cells during primitive erythropoiesis. Nature Commun 4: 2902, 2013
  • Yamazaki S, et al. A mouse model of adult-onset anaemia due to erythropoietin deficiency. Nature Commun 4, 1950 ,2013
  • Mitsuishi Y, et al. Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Cancer Cell 22, 66-79, 2012
  • Taguchi K, et al. Keap1 degradation by autophagy for the maintenance of redox homeostasis. Proc Natl Acad Sci USA 109, 13561-13566, 2012