Basic Medicine, Molecular and Cellular Biology

Molecular and Functional Dynamics

Development of Medical Technology for Overcoming Breast Cancer ~ Basic Research for Diagnosis and Therapy of Breast Cancer ~

Health Sciences Course

  • Master / Doctoral Degree

Faculty

HAYASHI, Shin-ichiHAYASHI, Shin-ichi
HAYASHI, Shin-ichi

Professor, Ph.D.

*Concurrent Position

Research Theme

  • Molecular mechanism of tumorigenesis and progression of hormone-dependent cancer
  • Mechanism of hormonal therapy-resistance and development of novel molecular target therapy
  • Development of new molecular biomarker and diagnosis for breast cancer
Research Keywords:

breast cancer, estrogen, hormonal therapy, molecular target therapy, nuclear receptor

Technical Keywords:

cell culture, molecular biology, biochemistry

Laboratory Introduction

The mission of our laboratory is education and research for the science underlying the field of clinical laboratory medicine: especially biochemistry, molecular biology, clinical chemistry, and analytical chemistry. Our main research projects are focused on steroid hormone-dependent tumors such as breast cancer. Recently, the number of breast cancer patients is rapidly increasing in Japan. The pathogenic mechanism is not fully understood. However, it is well known that female hormone, estrogen plays important roles. Estrogen is an important signaling molecule to regulate cell proliferation, differentiation, and homeostasis in various organs. On the other hand, estrogen acts for the occurrence and proliferation of breast and endometrial cancers. In these cancer cells, the pathogenesis is closely related to the nuclear estrogen receptor function. In fact, it is well known as hormone therapy, widely employed and show good efficacy in clinical treatment to block the function of estrogen receptor by the anti-hormonal agents.
We are studying in the molecular mechanisms of breast cancer progression, predicting the effect of hormone therapy, mechanisms of resistance to hormone therapy, breast cancer stem cell research. Our aim is to contribute to the patients with breast cancer and the society through these studies.

Figure 1. Mechanism of hormonal therapy for breast cancer and its resistance

Figure 1. Mechanism of hormonal therapy for breast cancer and its resistance

Figure 2. Visualized ER activity by GFP in breast cancer

Figure 2. Visualized ER activity by GFP in breast cancer

Recent Publications

  • Hanamura T, et al. Androgen metabolite-dependent growth of hormone receptor-positive breast cancer as a possible aromatase inhibitor-resistance mechanism. Breast Cancer Res. Treat. 139: 731-740, 2013
  • Fujiki N, et al. Estrogen response element-GFP (ERE-GFP) introduced MCF-7 cells demonstrated the coexistence of multiple estrogen-deprivation resistant mechanisms. J. Steriod Biochem. Mol. Biol., 139: 61-72, 2014
  • Hanamura T, et al. Possible role of the aromatase-independent steroid metabolism pathways in hormone responsive primary breast cancers. Breast Cancer Res. Treat. 143: 69-80, 2014
  • Yamaguchi Y, et al. Detection of estrogen-independent growth-stimulating activity in breast cancer tissues: implication for tumor aggressiveness. Cancer Microenvironment, 7: 23-21, 2014
  • Fujii R, et al. Increased androgen receptor activity and cell proliferation in aromatase inhibitor-resistant breast carcinoma. J. Steriod Biochem. Mol. Biol., 144: 513-522, 2014