Basic Medicine, Genomics, Genetics and Epigenetics, Oncology
Elucidation of Molecular Mechanisms of Cell Proliferation and Differentiation and Their System Failures, Unlock the Secret of Life
Medical Sciences Course
- Master / Doctoral Degree
- NAKAYAMA, Keiko
Professor, M.D. Ph.D.
nakayak2*med.tohoku.ac.jp (Please convert "*" into "@".)
- Alteration of genome and epigenome during oncogenesis and cellular abnormality consequent to oncogenesis
- Regulation of protein stability and elucidation of pathological condition caused by abnormality of protein stability
Oncogenesis, Epigenetics, Proteolysis
Next Generation Sequencer, Gene Engineering Mouse, Protein Chemistry, Cell Biology
We are interested in molecular mechanisms of cell cycle regulation by proteolysis, as well as epigenetic change during differentiation or oncogenesis.
We, multicellular organisms develop and differentiate from only one fertilized egg to adult tissues. During cell cycle, various regulatory proteins express in adequate amounts at the right time. This elaborate regulation partly depends on ubiquitin-proteasome system. We generate and analyze gene-engineered mouse related this system to elucidate the physiological functions of these molecules and biological and pathological significance of these molecules and system.
On another front, we address cell cycle and differentiation from the perspective of epigenetics. Our bodies are comprised of various kinds of cells. These cells share the same genome, but they function differently. It is suggested that diverse epigenomic profiles make cells function differently. We analyze epigenome comprehensively using the next-generation sequencer to understand what makes change epigenomic profiles and how maintain these profiles.
At the research field of life science and medicine, novel methods of study develop in rapid-fire succession, resulting in generation of many novel findings. We will learn and take these new methods and expand our view of research.
Figure 1. Effect of oncogene Ras on Histone H3K27me3
Figure 2. Expression profile of ABC transporter in normal colon tissue and colon cancer
- Hosogane M, et al. Lack of Transcription Triggers H3K27me3 Accumulation in the Gene Body. Cell Rep. 16(3):696-706, 2016
- Ishida N, et al. Ubiquitylation of Ku80 by RNF126 promotes completion of NHEJ-mediated DNA repair. Mol Cell Biol in press
- Kobayashi M, et al. Wnt-β-catenin signaling regulates ABCC3 (MRP3) transporter expression in colorectal cancer. Cancer Sci. 107(12):1776-1784, 2016
- Nakagawa T, et al. CRL4 E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases. Mol Cell 57: 247-260 2015
- Nakagawa, T., et al. S6 Kinaseand beta-TrCP2-Dependent Degradation of p19Arf Is Required for Cell Proliferation. Mol Cell Biol 35:3517-3527, 2015