Clinical Pharmaceutical Science
- MANO, Nariyasu
- SHIMADA, Miki
Our research field is the pharmaceutical care and the drug discovery using forefront techniques in bioanalytical chemistry, molecular biology, and pharmacokinetics.
1)Clinical and pharmaceutical application of proteomics and metabolomics
We currently study the basic and clinical methodology for proteomics and metabolomics to elucidate disease-relating proteins and bio-molecules, and their relationships.
2)Functional analysis of bio-molecules and clinical applications of the methods
We develop unique methods for the identification of proteins bound to bio-molecules and their complex. This method is very useful to know the pharmacological and side effects of the medicines and their candidates.
3)Application of analytical methods for identification of drugs and endogenous compounds
Based on the chromatographic and mass spectrometric analysis, rapid and sensitive methods to quantify a number of medicines (anti-cancer, immunosuppressant, antibacterial drugs etc.) have been established. This method is successfully applicable to therapeutic drug monitoring in blood from patients.
4)Analysis of genetic polymorphisms influencing drug effects
After determination of the factors influencing drug effects, such as transporters, and drug-metabolizing enzymes, we develop rapid- and high-sensitive methods for the genotyping of polymorphisms influencing drug response and toxicity. These pharmacogenomic studies may contribute to providing a personalized medicine for each individual in the future.
Selected Awards, articles and books
1)Rapid screening of antineoplastic candidates for the human organic anion transporter OATP1B3 substrates using fluorescent probes.Cancer Lett., 260, 163-169, 2008
2)Specific affinity extraction method for small molecule-binding proteins. Anal. Chem., 78, 4668-4675, 2006
3)Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3.J. Lipid Res., 47, 1196-1202, 2006
4)A nonenzymatic modification of the amino-terminal domain of histone H3 by bile acid acyl adenylate. J. Biol. Chem., 279, 55034-55041, 2004
5)Presence of protein-bound unconjugated bile acids in the cytoplasmic fraction of rat brain.J. Lipid Res., 45, 295-300, 2004
bioanalytical chemistry, pharmacokinetics, molecular biology, metabolomics, proteomics, bile acid, drug metabolizing enzymes, transporter, polymorphysm, bio-molecules
- shimada*m.tains.tohoku.ac.jp(Please convert "*" into "@".)
- TEL +81-22-717-7541
- FAX +81-22-717-7545