Basic Medicine, Bioinformatics, Genomics, Genetics and Epigenetics
Genomic Imprinting in Mammals and Evolution
Medical Sciences Course
- Master / Doctoral Degree
- ARIMA, Takahiro
Professor, M.D. Ph.D.
tarima*med.tohoku.ac.jp (Please convert "*" into "@".)
- Genomic imprinting and mammalion evolution
- Molecular mechanism of genomic imprinting phenomenon
- Study on association between human assisted reproductive technology (ART) and congenital imprinting diseases
Genomic imprinting, Epigenetics, Placenta, Assisted reproductive technology (ART), Evolution
DNA methylation, Histone modification , RNA sequence
Our aim is to determine the normal epigenomes of the human placenta, which constitute the fetal-maternal interface, for future use in studies of human diseases associated with reproduction and development. In the placenta, we focus on cytotrophoblast and syncytiotrophoblast cells. To this end, we will develop and establish the technologies to isolate these cells and analyze the epigenomic modifications. We will further try to determine the epigenomes of disease samples, to search for disease-specific changes. We will also determine the methylomes of sperm from oligospermic patients for their use in the improvement of assisted reproductive technology.
Figure 1. Incomplete erasure of germline DNA methylation in the human placenta
Figure 2. HTS cells
- Okae H, et al. Genome-wide analysis of DNA methylation dynamics during early human development. PLOS Genetics. 10(12): e1004868, 2014.
- Okae H, et al. RNA sequencing-based identification of aberrant imprinting in cloned mice. Hum Mol Genet.23. 992-1001. 2014.
- Okae H, et al. Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression. Hum Mol Genet. 21: 548-558, 2012.
- Watanabe T, et al. Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus. Science. 332: 848-852, 2011.
- Borgel, J., et al. Targets and dynamics of promoter DNA methylation during early mouse development. Nat. Genet. 42: 1093-1100, 2010.