東北大学大学院医学系研究科・医学部

STAFF

Professor

• Katsuoka, FumikiProfessor. 勝岡 史城 教授
  

Other Faculty / Staff

• Hidaka, Takanori

  Assistant Prof. 日高 高徳 助教

CONTACT

OUTLINE

The genomic information encoded in the DNA sequences defines the cellular differentiation and homeostasis through transcription and translation. Our lab have been studying the mechanism of transcriptional regulation, focusing on the small Maf (sMaf) transcription factor family members, which play important roles in the expression of cytoprotective genes. The sMaf proteins form various heterodimers with CNC and Bach transcription factors, and positively or negatively regulate transcription through DNA sequences called CsMBE (CNC-sMaf binding elements). While a variety of CNC-sMaf heterodimers play roles in cells, the target gene selection specificity of each dimer is not fully understood. In addition, while three sMaf proteins, MafF, MafG, and MafK, play roles in vertebrates, but the differences in their functions are not clear. The aim of our research is to elucidate the molecular basis for the regulation of gene expression by the CNC-sMaf dimer.Please refer to the Wikipedia article on “sMaf proteins”page(URL: wikipedia.org/wiki/Small_Maf), which we edited.
We are also working on the development of genome analysis methods using next-generation sequencers, and are supporting genome analysis projects conducted by the Tohoku Medical Megabank Organization and sequencing projects with clinical samples.

DNA配列に刻まれたゲノム情報は、転写、翻訳を経て、細胞の分化や恒常性を規定します。当分野では、各種ストレスに対する遺伝子の転写応答で重要な機能を担う小Maf群因子（sMaf）の機能を研究しています。sMafは、MafF, MafG, MafKの3種類があり、それぞれCNC・ Bach群因子と二量体を形成し、CNC-小Maf結合エレメント（CsMBE）を介して転写を制御します。しかし、様々なCNC-sMaf二量体の標的遺伝子選択特異性や、3種類の小Maf群因子の機能の差異は解明されていません。このCNC-sMaf二量体が規定する高次の遺伝子発現制御の分子基盤を明らかにすることが、当分野の研究目的です。当分野がまとめたsMafに関するWikipedia (URL: wikipedia.org/wiki/Small_Maf) もご参照下さい。
　上記のプロジェクトの他、次世代シークエンサーを活用したゲノム解析手法の開発にも取り組んでおり、東北メディカル・メガバンク機構が実施するゲノム解析プロジェクト、臨床検体を用いたクリニカルシークエンス等のプロジェクトを支援しています

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  Transcriptional regulation mediated by CNC-sMaf heterodimers
  CNC-小Mafヘテロ二量体による遺伝子発現制御

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  Next-generation sequencers
  次世代シークエンサー

ARTICLE

Katsuoka F, Otsuki A, Hatanaka N, Okuyama H, Yamamoto. M. Target Gene Diversity of the Nrf1-MafG Transcription Factor Revealed by a Tethered Heterodimer. Mol Cell Biol 42: e0052021, 2022
URL：https://pubmed.ncbi.nlm.nih.gov/35129372/

Takayama J, Tadaka S, Yano K, Katsuoka F, et al. Construction and integration of three de novo Japanese human genome assemblies toward a population-specific reference. Nat Commun . 12: 226, 2021
URL：https://pubmed.ncbi.nlm.nih.gov/33431880/

Otsuki A, Okamura Y, Aoki Y, Ishida N, Kumada K, Minegishi N, Katsuoka F, Kinoshita K, Yamamoto M. Identification of Dominant Transcripts in Oxidative Stress Response by a Full-Length Transcriptome Analysis. Mol Cell Biol. 41: e00472-20, 2021
URL：https://pubmed.ncbi.nlm.nih.gov/33168698/

Katsuoka F, Otsuki A, Takahashi M, Ito S, Yamamoto M. Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells. Mol Cell Biol. 39: e00273-19, 2019
URL：https://pubmed.ncbi.nlm.nih.gov/31383749/

Ishida N, Aoki Y, Katsuoka F, et al. Landscape of electrophilic and inflammatory stress-mediated gene regulation in human lymphoblastoid cell lines. Free Radic Biol Med. 161:71-83, 2020
URL：https://pubmed.ncbi.nlm.nih.gov/33011271/